Biosynthesis of heparin. Enzymatic sulfation of pentasaccharides.

Enzymatic sulfation of chondroitin B.

Enzymatic Sulfation of Mucopolysaccharides in Hen Oviduct

An enzymatic reaction has been identified in the isthmus of hen oviduct in which sulfate is transferred from 3’-phosphoadenosine 5’-phosphosulfate to mucopolysaccharide acceptors (1). From studies of the reaction with monosaccharides and oligosaccharides as model acceptors (‘2) it was concluded that one or more enzymes in the preparations employed catalyze a direct transfer of sulfate from PAPS...

Biosynthesis of chondroitin sulfate. Organization of sulfation.

The potential relationship of an intact membrane organization for the synthesis of chondroitin and chondroitin 4-sulfate was examined after modification of a mouse mast cell microsomal system with the nonionic detergent, Triton X-100. The results indicated that Triton X-100 had no effect on the rate of polymerization but had a slight effect on the size of glycosaminoglycan chains. An "all or no...

Biosynthesis of Heparin

Microsomal fraction from mouse mastocytoma was incubated for 60 min with UDP-[Wlglucuronic acid and unlabeled UDP-N-acetylglucosamine, producing nonsulfated labeled polysaccharide. Continued incubation for an additional 60-min period, in the presence of 3’-phosphoadenylylsulfate, yielded sulfated, heparin-like polymer. The incorporation of W was terminated at the beginning of the sulfation peri...

Biosynthesis of Heparin

Microsomal fraction from mouse mastocytoma was incubated for 60 min with UDP-[Wlglucuronic acid and unlabeled UDP-N-acetylglucosamine, producing nonsulfated labeled polysaccharide. Continued incubation for an additional 60-min period, in the presence of 3’-phosphoadenylylsulfate, yielded sulfated, heparin-like polymer. The incorporation of W was terminated at the beginning of the sulfation peri...